2018

Original articles

Ochirkhuu N, Konnai S, Odbileg R, Okagawa T, Murata S, Ohashi K. Identification of immuno-inhibitory molecules in Mongolian native cattle and yak. Jpn. J. Vet. Res. 66(3):177-192.

 

Mekata H, Yamamoto M, Hayashi T, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J. 2018. Cattle with a low bovine leukemia virus proviral load are rarely an infectious source. Jpn. J. Vet. Res. 66(3):157-163.

 

Tagawa M, Kurashima C, Takagi S, Maekawa N, Konnai S, Shimbo G, Matsumoto K, Inokuma H, Kawamoto K, Miyahara K. 2018. Evaluation of costimulatory molecules in dogs with B cell high grade lymphoma. PLoS One. 13(7):e0201222. doi: 10.1371/journal.pone.0201222.

 

Yamada S, Itai S, Kaneko MK, Konnai S, Kato Y. 2018. Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1. Biochem. Biophys. Rep. 15:52-56. doi: 10.1016/j.bbrep.2018.07.002.

 

Okagawa T, Konnai S, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Kohara J, Suzuki Y, Yamada S, Kato Y, Murata S, Ohashi K. Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection. Vet. Res. 49:50. doi: 10.1186/s13567-018-0543-9.

 

Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Nagata R, Kawaji S, Kagawa Y, Yamada S, Kato Y, Nakajima C, Suzuki Y, Murata S, Mori Y, Ohashi K. 2018. Prostaglandin E2 induction suppresses the Th1 immune responses in cattle with Johne's disease. Infect. Immun. 86(5):e00910-17. doi: 10.1128/IAI.00910-17.

 

Mekata H, Yamamoto M, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J. 2018. New hematological key for bovine leukemia virus-infected Japanese Black cattle. J. Vet. Med. Sci. 80(2):316-319. doi: 10.1292/jvms.17-0455. Epub 2018 Jan 22.

 

Seixas A, Alzugaray MF, Tirloni L, Parizi LF, Pinto AFM, Githaka NW, Konnai S, Ohashi K, Yates JR III, Termignoni C, da Silva Vaz I Jr. 2018. Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis. Ticks Tick Borne Dis. 9(1):72-81. doi: 10.1016/j.ttbdis.2017.10.006. Epub 2017 Oct 16.

 


2017

Original articles

Sajiki Y, Konnai S, Nishimori A, Okagawa T, Maekawa N, Goto S, Nagano M, Kohara J, Kitano N, Takahashi T, Tajima M, Mekata H, Horii Y, Murata S, Ohashi K. 2017. Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load. J. Vet. Med. Sci. 79:2036-2039. doi: 10.1292/jvms.17-0391. Epub 2017 Nov 6.

 

Itai S, Yamada S, Kaneko MK, Harada H, Kagawa Y, Konnai S, Kato Y. 2017. Expression of Cat Podoplanin in Feline Squamous Cell Carcinomas. Monoclon. Antib. Immunodiagn. Immunother. 36:243-250. doi: 10.1089/mab.2017.0046. Epub 2017 Nov 1.

 

Ochirkhuu N, Konnai S, Odbileg R, Murata S, Ohashi K. 2017. Molecular epidemiological survey and genetic characterization of ovine gammaherpesvirus-2 in Mongolian livestock. J. Vet. Med. Sci. 79:2040-2042. doi: 10.1292/jvms.17-0203. Epub 2017 Oct 19.

 

Sharav T, Konnai S, Ochirkhuu N, Ts EO, Mekata H, Sakoda Y, Umemura T, Murata S, Chultemdorj T, Ohashi K. 2017. Detection and molecular characterization of equine infectious anemia virus in Mongolian horses. J. Vet. Med. Sci. 79:1884-1888. doi: 10.1292/jvms.17-0202. Epub 2017 Oct 11.

 

Maekawa N, Konnai S, Balbin MM, Mingala CN, Gicana KRB, Bernando FAEM, Murata S, Ohashi K. 2017. Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog. Ticks Tick Borne Dis9:266-269. doi: 10.1016/j.ttbdis.2017.09.012. Epub 2017 Sep 20.

 

Maekawa, N., Konnai, S., Takagi, S., Kagawa, Y., Okagawa, T., Nishimori, A., Ikebuchi, R., Izumi, Y., Deguchi, T., Nakajima, C., Kato, Y., Yamamoto, K., Uemura, H., Suzuki, Y., Murata, S. and Ohashi K. 2017. A canine chimeric monoclonal antibody targeting PD-L1 and its clinical efficacy in canine oral malignant melanoma or undifferentiated sarcoma. Sci. Rep. 7:8951. (doi: 10.1038/s41598-017-09444-2.)

 

Yamada, S., Itai, S., Nakamura, T., Yanaka, M., Saidoh, N., Chang, Y. W., Handa, S., Harada, H., Kagawa, Y., Ichii, O., Konnai, S., Kaneko, M. K. and Kato, Y. 2017. PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother. 36:224-230. doi: 10.1089/mab.2017.0027. Epub 2017 Jul 24.

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Murata, S. and Ohashi, K. 2017. Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock. Vector Borne Zoonotic Dis. 17:539-549. (doi:10.1089/vbz.2017.2111.)

 

Nishimori, A., Konnai, S., Okagawa, T., Maekawa, N., Goto, S., Ikebuchi, R., Nakahara, A., Chiba, Y., Ikeda, M., Murata, S. and Ohashi, K. 2017. Identification of an atypical enzootic bovine leukosis in Japan by using a novel classification of bovine leukemia based on immunophenotypic analysis. Clin. Vaccine Immunol. 24(9):e00067-17. (doi: 10.1128/CVI.00067-17.)

 

Okagawa, T., Konnai, S., Nishimori, A., Maekawa, N., Ikebuchi, R., Goto, S., Nakajima, C., Kohara, J., Ogasawara, S., Kato, Y., Suzuki, Y., Murata, S. and Ohashi, K. 2017. Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Front. Immunol. 8:650. (doi:10.3389/fimmu.2017.00650.)

 

Goto, S., Konnai, S., Okagawa, T., Nishimori, A., Maekawa, N., Gondaira, S., Higuchi, H., Koiwa, M., Tajima, M., Kohara, J., Ogasawara, S., Kato, Y., Suzuki, Y., Murata, S. and Ohashi, K. 2017. Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis. Immun. Inflamm. Dis. 5:355-363. doi: 10.1002/iid3.173. Epub 2017 May 24.

 

Yamada, S., Honma, R., Kaneko, M. K., Nakamura, T., Yanaka, M., Saidoh, N., Takagi, M., Konnai, S. and Kato, Y. 2017. Characterization of the Anti-Bovine Podoplanin Monoclonal Antibody PMab-44. Monoclon. Antib. Immunodiagn. Immunother. 36: 129-134. (doi:10.1089/mab.2017.0016.)

 

Nishimori, A., Konnai, S., Okagawa, T., Maekawa, N., Ikebuchi, R., Goto, S., Sajiki, Y., Suzuki, Y., Kohara, J., Ogasawara, S., Kato, Y., Murata, S. and Ohashi, K. 2017. In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection. PLoS One. 12: e0174916. (doi: 10.1371/journal.pone.0174916.)

 

Yamada, S., Kaneko, M. K., Nakamura, T., Ichii, O., Konnai, S. and Kato, Y. 2017. Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin. Monoclon. Antib. Immunodiagn. Immunother. 36: 77-79. (doi: 10.1089/mab.2017.0002.)

 

Rangel, C. K., Parizi, L. F., Sabadin, G. A., Costa, E.P., Romeiro, N. C., Isezaki, M., Githaka, N.W., Seixas, A., Logullo, C., Konnai, S., Ohashi, K. and da Silva Vaz I. Jr. 2017. Molecular and structural characterization of novel cystatins from the taiga tick Ixodes persulcatus. Ticks Tick Borne Dis. 8: 432-441. (doi:10.1016/j.ttbdis.2017.01.007.)

 

Pandey, G. S., Simulundu, E., Mwiinga, D., Samui, K. L., Mweene, A. S., Kajihara, M., Mangani, A., Mwenda, R., Ndebe, J., Konnai, S. and Takada, A. 2017. Clinical and subclinical bovine leukemia virus infection in a dairy cattle herd in Zambia. Arch. Virol. 162: 1051-1056. (doi: 10.1007/s00705-016-3205-0.)

 

Konnai, S., Murata, S. and Ohashi, K. 2017. Immune exhaustion during chronic infections in cattle. J. Vet. Med. Sci. 79: 1-5. (doi: 10.1292/jvms.16-0354.)

 

Costa, E. P., Façanha, A. R., Cruz, C. S., Silva, J. N., Machado, J. A., Carvalho, G. M., Fernandes, M. R., Martins, R., Campos, E., Romeiro, N. C., Githaka, N. W., Konnai, S., Ohashi, K., Vaz I. S. Jr. and Logullo, C. 2017. A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase. Biochim. Biophys. Acta. 1861(1 Pt A): 2922-2933. (doi: 10.1016/j.bbagen.2016.09.017.)

 

Machida, Y., Murata, S., Matsuyama-Kato, A., Isezaki, M., Taneno, A., Sakai, E., Konnai, S. and Ohashi, K. 2017. Isolation and purification of Gallid herpesvirus 2 strains currently distributed in Japan. J. Vet. Med. Sci. 79: 115-122. (doi:10.1292/jvms.16-0329.)

 

Text

岡川朋弘, 他. 2017. 実験医学別冊 ラボ必携フローサイトメトリーQ&A. 羊土社.

 

岡川朋弘, 今内 覚 他. 2017. 動物用ワクチンとバイオ医薬品 -新たな潮流-. 文永堂出版.

 


2016

Original articles

Ogasawara, S., Honma, R., Kaneko, M. K., Fujii, Y., Kagawa, Y., Konnai, S. and Kato, Y. 2016. Podoplanin Expression in Canine Melanoma. Monoclon. Antib. Immunodiagn. Immunother. 35: 304-306. (doi: 10.1089/mab.2016.0040.)

 

Kaneko, M. K., Honma, R., Ogasawara, S., Fujii, Y., Nakamura, T., Saidoh, N., Takagi, M., Kagawa, Y., Konnai, S. and Kato, Y. 2016. PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas. Monoclon. Antib. Immunodiagn. Immunother. 35: 263-266.

 

Okagawa, T., Konnai, S., Deringer, J. R., Ueti, M. W., Scoles, G. A., Murata, S., Ohashi, K. and Brown, W. C. 2016. Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle. Infect. Immun. 84: 2779-2790. (doi: 10.1128/IAI.00278-16.)

 

Honma, R., Kaneko, M. K., Ogasawara, S., Fujii, Y., Konnai, S., Takagi, M. and Kato, Y. 2016. Specific Detection of Dog Podoplanin Expressed in Renal Glomerulus by a Novel Monoclonal Antibody PMab-38 in Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother. 35: 212-216. (doi: 10.1089/mab.2016.0022.)

 

Maekawa, N., Konnai, S., Okagawa, T., Nishimori, A., Ikebuchi, R., Izumi, Y., Takagi, S., Kagawa, Y., Nakajima, C., Suzuki, Y., Kato, Y., Murata, S. and Ohashi, K. 2016. Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma. PLoS One. 11: e0157176. (doi: 10.1371/journal.pone.0157176.)

 

Honma, R., Ogasawara, S., Kaneko, M. K., Fujii, Y., Oki, H., Nakamura, T., Takagi, M., Konnai, S. and Kato, Y. 2016. PMab-44 Detects Bovine Podoplanin in Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother. [Epub ahead of print]

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Odzaya, B., Gansukh, S., Murata, S. and Ohashi, K. 2016. Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks. Arch. Virol. 161: 2279-2283. (doi: 10.1007/s00705-016-2890-z.)

 

Ohira, K., Nakahara, A., Konnai, S., Okagawa, T., Nishimori, A., Maekawa, N., Ikebuchi, R., Kohara, J., Murata, S. and Ohashi, K. 2016. Bovine leukemia virus reduces anti-viral cytokine activities and NK cytotoxicity by inducing TGF-β secretion from regulatory T cells. Immun. Inflamm. Dis. 4: 52-63. (doi: 10.1002/iid3.93.)

 

Honma, R., Fujii, Y., Ogasawara, S., Oki, H., Konnai, S., Kagawa, Y., Takagi, M., Kaneko, M. K. and Kato, Y. 2016. Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis. Monoclon. Antib. Immunodiagn. Immunother. 35: 65-72. (doi: 10.1089/mab.2015.0078.)

 

Nishimori, A., Konnai, S., Ikebuchi, R., Okagawa, T., Nakahara, A., Murata, S. and Ohashi, K. 2016. Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection. J. Vet. Med. Sci. 78: 791-796. (doi: 10.1292/jvms.15-0577.)

 

Tagawa, M., Maekawa, N., Konnai, S. and Takagi, S. 2016. Evaluation of Costimulatory Molecules in Peripheral Blood Lymphocytes of Canine Patients with Histiocytic Sarcoma. PLoS One. 11: e0150030. (doi: 10.1371/journal.pone.0150030.)

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Nishimori, A., Okagawa, T., Murata, S. and Ohashi, K. 2016. Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle. Arch. Virol. 161: 985-991. (doi: 10.1007/s00705-015-2676-8.)

 

Toyomane, K., Konnai, S., Niwa, A., Githaka, N., Isezaki, M., Yamada, S., Ito, T., Takano, A., Ando, S., Kawabata, H., Murata, S. and Ohashi, K. 2016. Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze. Ticks Tick Borne Dis. 7: 119-125. (doi: 10.1016/j.ttbdis.2015.09.006.)

 

今内 覚, 村田史郎, 大橋和彦. 2016. 牛白血病への対策を練る〜牛白血病について〜. 養牛の友. 7: 32-36.

 

今内 覚. 2016. 初乳の衛生管理について 〜健康な子牛を育てるために:牛白血病防除の観点から〜. 乳房炎防除対策研究会誌. 22: 21-23.

 

今内 覚. 2016. ウシ慢性感染症における免疫応答の解析と新規制御法への応用. 畜産技術. 2: 2-5.

 

今内 覚, 村田史郎, 大橋和彦. 2016. 増加する牛白血病とその対策. 農家の友. 68: 108-110.

 

今内 覚. 2016. Programmed death-1 (PD-1). Veterinary Oncology. 3: 12-14.

 

今内 覚. 2016.牛白血病防除の観点から. 検定検査乳S. 34:7.

 


2015

Original articles

Ochirkhuu, N., Konnai, S., Odbileg, R., Murata, S. and Ohashi, K. 2015. A preliminary survey of the seroprevalence of Mycobacterium avium subspecies paratuberculosis in Mongolian cattle. Jpn. J. Vet. Res. 63: 191-194.

 

Okagawa, T., Konnai, S., Nishimori, A., Ikebuchi, R., Mizorogi, S., Nagata, R., Kawaji, S., Tanaka, S., Kagawa, Y., Murata, S., Mori, Y. and Ohashi, K. 2015. Bovine Immunoinhibitory Receptors Contribute to Suppression of Mycobacterium avium subsp. paratuberculosis-Specific T-Cell Responses. Infect. Immun. 84: 77-89. (doi: 10.1128/IAI.01014-15.)

 

Duran, P. L., Padiernos, R. B., Abella, E. A., Konnai, S. and Mingala, C. N. 2015. Molecular characterization of T-cell immunoglobulin mucin domain-3 and Galectin-9 genes of swamp- and riverine-type water buffaloes. Int. J. Immunogenet. 42: 469-78. (doi: 10.1111/iji.12235.)

 

Ochirkhuu, N., Konnai, S., Mingala, C. N., Okagawa, T., Villanueva, M., Pilapil, F. M., Murata, S. and Ohashi, K. 2015. Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon Island, the Philippines. Vet. Parasitol. 212: 161-167. (doi: 10.1016/j.vetpar.2015.05.019.)

 

Gomes, H., Moraes, J., Githaka, N., Martins, R., Isezaki, M., Vaz, Ida. S. Jr., Logullo, C., Konnai, S. and Ohashi, K. 2015. Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation. Vet. Parasitol. 211: 266-273. (doi: 10.1016/j.vetpar.2015.05.022.)

 

Nakata, H., Nakayama, S. M., Ikenaka, Y., Mizukawa, H., Ishii, C., Yohannes, Y. B., Konnai, S., Darwish, W. S. and Ishizuka, M. 2015. Metal extent in blood of livestock from Dandora dumping site, Kenya: Source identification of Pb exposure by stable isotope analysis. Environ. Pollut. 205: 8-15. (doi: 10.1016/j.envpol.2015.05.003.)

 

Mekata, H., Sekiguchi, S., Konnai, S., Kirino, Y., Horii, Y. and Norimine, J. 2015. Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan. J. Vet. Med. Sci. 77: 1115-1120. (doi: 10.1292/jvms.14-0624.)

 

Parizi, L. F., Sabadin, G. A., Alzugaray, M. F., Seixas, A., Logullo, C., Konnai, S., Ohashi, K., Masuda, A. and da Silva, Vaz, I. Jr. 2015. Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response. Parasit. Vectors. 8: 122. (doi: 10.1186/s13071-015-0743-3.)

 

Mekata, H., Murata, S., Mingala, C. N., Ohashi, K. and Konnai, S.2015. Expression of regulatory dendritic cell-related cytokines in cattle experimentally infected with Trypanosoma evansi. J. Vet. Med. Sci. 77: 1017-1019. (doi: 10.1292/jvms.15-0066.)

 

Gondaira, S., Higuchi, H., Iwano, H., Nakajima, K., Kawai, K., Hashiguchi, S., Konnai, S. and Nagahata, H. 2015. Cytokine mRNA profiling and the proliferative response of bovine peripheral blood mononuclear cells to Mycoplasma bovis. Vet. Immunol. Immunopathol. 165: 45-53. (doi: 10.1016/j.vetimm.2015.03.002.)

 

Murase, Y., Konnai, S., Yamada, S., Githaka, N., Isezaki, M., Ito, T., Takano, A., Ando, S., Kawabata, H., Murata, S. and Ohashi, K. 2015. An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes. Insect Biochem. Mol. Biol. 60: 59-67. (doi: 10.1016/j.ibmb.2015.01.010.)

 

Suzuki, S., Konnai, S., Okagawa, T., Ikebuchi, R., Nishimori, A., Kohara, J., Mingala, C. N., Murata, S. and Ohashi, K. 2015. Increased expression of the regulatory T cell-associated marker CTLA-4 in bovine leukemia virus infection. Vet. Immunol. Immunopathol. 163: 115-124. (doi: 10.1016/j.vetimm.2014.10.006.)

 

da Silva, R. M., Noce, B. D., Waltero, C. F., Costa, E. P., de Abreu, L. A., Githaka, N. W., Moraes, J., Gomes, H. F., Konnai, S., Vaz, Ida, S. Jr., Ohashi, K. and Logullo, C. 2015. Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26. Int. J. Mol. Sci. 16: 1821-1839. (doi: 10.3390/ijms16011821.)

 

Mekata, H., Sekiguchi, S., Konnai, S., Kirino, Y., Honkawa, K., Nonaka, N., Horii, Y. and Norimine, J. 2015. Evaluation of the natural perinatal transmission of bovine leukaemia virus. Vet. Rec. 176: 254. (doi: 10.1136/vr.102464.)

 

今内 覚. 2015. 牛白血病における免疫応答. 産業動物臨床医学雑誌. 6: 130-132.

 

今内 覚. 2015. 牛白血病の現状と対策の例. 宮城県獣医師会会報. 68: 187-193.

 

今内 覚 他. 2015. 牛の感染症の対策・防除を考える. 臨床獣医. 33: 11-39.

 

今内 覚. 2015. 抗体医薬による免疫療法. 動物用ワクチンーバイオ医薬品研究会ニュースレター. 11: 1-2.

 

今内 覚. 2015. 牛白血病 最近の知見と対策について. 動薬研究. 6: 1-11.

 

今内 覚. 2015. 現代農業・牛白血病を出さない!尾鈴のBL対策. 宮崎県JA尾鈴農山漁村文化協会.

 

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今内 覚 他. 2015. 獣医免疫学. 緑書房.

 


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