2024

Original articles

Nao N, Okagawa T, Nojiri N, Konnai S, Shimakura H, Tominaga M, Yoshida-Furihata H, Nishiyama E, Matsudaira T, Maekawa N, Murata S, Muramatsu M, Ohashi K, Saito M. 2024. Chimeric provirus of bovine leukemia virus/SMAD family member 3 in cattle with enzootic bovine leukosis. Arch Virol., 169(3):47. doi: 10.1007/s00705-024-05970-3

 

Win SY, Seo H, Horio F, Fujisawa S, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawn S, Okagawa T, Maekawa N, Konnai S, Ohashi K, Murata S. 2024. In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model. Vaccines, 12(2):148. doi: 10.3390/vaccines12020148

 

Motai Y, Murata S, Sato J, Nishi A, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2024. Characterization of a Very Short Meq Protein Isoform in a Marek’s Disease Virus Strain in Japan. Vet. Sci., 11(1):43. doi: 10.3390/vetsci11010043

 

 

2023

Original articles

Berger M, Rosa da Mata S, Pizzolatti NM, Parizi LF, Konnai S, da Silva Vaz I Jr, Seixas A, Tirloni L. 2023. An Ixodes persulcatus inhibitor of plasmin and thrombin hinders keratinocyte migration, blood coagulation, and endothelial permeability. J. Invest. Dermatol., in press. doi: 10.1016/j.jid.2023.10.026

 

Moraes B, Martins R, Lopes C, Martins R, Arcanjo A, Nascimento J, Konnai S, da Silva Vaz I Jr, Logullo C. 2023. G6PDH as a key immunometabolic and redox trigger in arthropods. Front. Physiol., 14:1287090. doi: 10.3389/fphys.2023.1287090

 

Maekawa N, Konnai S, Hosoya K, Kim S, Kinoshita R, Deguchi T, Owaki R, Tachibana Y, Yokokawa M, Takeuchi H, Kagawa Y, Takagi S, Ohta H, Kato Y, Yamamoto S, Yamamoto K, Suzuki Y, Okagawa T, Murata S, Ohashi K. 2023. Safety and clinical efficacy of an anti-PD-L1 antibody (c4G12) in dogs with advanced malignant tumours. PLoS One, 18(10):e0291727. doi: 10.1371/journal.pone.0291727

 

Okagawa T, Konnai S, Goto S, Sajiki Y, Ganbaatar O, Watari K, Nakamura H, Wang CX, Tachibana T, Kato Y, Kameda Y, Kohara J, Terasaki N, Kubota M, Takeda A, Takahashi H, Suzuki Y, Maekawa N, Murata S, Ohashi K. 2023. Development of a high-affinity anti-bovine PD-1 rabbit-bovine chimeric antibody using an efficient selection and large production system. Vet. Res., 54(1):82. doi: 10.1186/s13567-023-01213-6

 

Win SY, Murata S, Fujisawa S, Seo H, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawm S, Okagawa T, Maekawa N, Konnai S, Ohashi K. 2023. Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species. PLoS One, 18(7):e0288565. doi: 10.1371/journal.pone.0288565

 

Deguchi T, Maekawa N, Konnai S, Owaki R, Hosoya K, Morishita K, Nakamura M, Okagawa T, Takeuchi H, Kim S, Kinoshita R, Tachibana Y, Yokokawa M, Takagi S, Kato Y, Suzuki Y, Murata S, Ohashi K. 2023. Enhanced Systemic Antitumour Immunity by Hypofractionated Radiotherapy and Anti-PD-L1 Therapy in Dogs with Pulmonary Metastatic Oral Malignant Melanoma. Cancers, 15(11):3013. doi: 10.3390/cancers15113013

 

Tiyamanee W, Konnai S, Okagawa T, Nojima Y, Ganbaatar O, Maekawa N, Hasebe R, Kagawa Y, Kato Y, Suzuki Y, Murata S, Ohashi K. 2023. Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in sheep. Vet. Immunol. Immunopathol.,  261:110609. doi: 10.1016/j.vetimm.2023.110609

 

Win SY, Murata S, Fujisawa S, Seo H, Sato J, Motai Y, Sato T, Oishi E, Taneno A, Htun LL, Bawm S, Okagawa T, Maekawa N, Konnai S, Ohashi K. 2023. Potential of ferritin 2 as an antigen for the development of a universal vaccine for avian mites, poultry red mites, tropical fowl mites, and northern fowl mites. Front. Vet. Sci., 10:1182930. doi: 10.3389/fvets.2023.1182930

 

Ikehata M, Konnai S, Okagawa T, Abe K, Honma M, Kitamura T, Maekawa N, Suzuki Y, Murata S, Ohashi K. 2023. In vitro evaluation of Lactiplantibacillus plantarum HOKKAIDO strain, effective lactic acid bacteria for calf diarrhea. Front. Vet. Sci., 10:1145445. doi: 10.3389/fvets.2023.1145445

 

Fujisawa S, Murata S, Sato T, Oishi E, Taneno A, Konnai S, Ohashi K. 2023.Investigation of peripheral blood responses in chickens infested with Dermanyssus gallinae. Parasitol. Int., 95:102754. doi: 10.1016/j.parint.2023.102754

 

Okagawa T, Konnai S, Nakamura H, Ganbaatar O, Sajiki Y, Watari K, Noda H, Honma M, Kato Y, Suzuki Y, Maekawa N, Murata S, Ohashi K. 2023. Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves. Vaccines, 11(3):559. doi: 10.3390/vaccines11030559

 

Seo H, Murata S, Ichii O, Namba T, Win SY, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2023. Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization. J. Vet. Med. Sci., 85(5):532-535. doi: 10.1292/jvms.22-0544

 

Owaki R, Deguchi T, Konnai S, Maekawa N, Okagawa T, Hosoya K, Kim S, Sunaga T, Okumura M. 2023. Regulation of programmed death ligand 1 expression by interferon-γ and tumour necrosis factor-α in canine tumour cell lines. Vet. Comp. Oncol., 21(2):279-290. doi: 10.1111/vco.12886

 

Fujisawa S, Murata S, Isezaki M, Win SY, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2023. Suppressive modulation of host immune responses by Dermanyssus gallinae infestation. Poult. Sci., 102(4):102532. doi: 10.1016/j.psj.2023.102532

 

Takeuchi H, Nakajima C, Konnai S, Maekawa N, Okagawa T, Usui M, Tamura Y, Suzuki Y, Murata S, Ohashi K. 2023. Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies. PLoS One, 18(1):e0281171. doi: 10.1371/journal.pone.0281171

 

Maekawa N, Konnai S, Asano Y, Otsuka T, Aoki E, Takeuchi H, Kato Y, Kaneko MK, Yamada S, Kagawa Y, Nishimura M, Takagi S, Deguchi T, Ohta H, Nakagawa T, Suzuki Y, Okagawa T, Murata S, Ohashi K. 2023. Molecular characterization of feline immune checkpoint molecules and establishment of PD-L1 immunohistochemistry for feline tumors. PLoS One, 18(1):e0281143. doi: 10.1371/journal.pone.0281143

 

Nakamura H, Konnai S, Okagawa T, Maekawa N, Sajiki Y, Watari K, Kamitani K, Saito M, Kato Y, Suzuki Y, Murata S, Ohashi K. 2023. Combined Immune Checkpoint Blockade Enhances Antiviral Immunity against Bovine Leukemia Virus. J. Virol., 97(1):e0143022. doi: 10.1128/jvi.01430-22

 

 

2022

Original articles

Okagawa T, Shimakura H, Konnai S, Saito M, Matsudaira T, Nao N, Yamada S, Murakami K, Maekawa N, Murata S, Ohashi K. 2022. Diagnosis and Early Prediction of Lymphoma Using High-Throughput Clonality Analysis of Bovine Leukemia Virus-Infected Cells. Microbiol. Spectr., 10(6):e0259522. doi: 10.1128/spectrum.02595-22

 

Sajiki Y, Konnai S, Watari K, Okagawa T, Tanaka A, Kawaji S, Nagata R, Maekawa N, Suzuki Y, Kato Y, Murata S, Mori Y, Ohashi K. 2022. Prostaglandin E2-Induced Immune Suppression via Cytotoxic T-Lymphocyte Antigen 4 in Paratuberculosis. Infect. Immun., 14:e0021022. doi: 10.1128/iai.00210-22

 

Maekawa N, Konnai S, Asano Y, Sajiki Y, Deguchi T, Okagawa T, Watari K, Takeuchi H, Takagi S, Hosoya K, Kim S, Ohta H, Kato Y, Suzuki Y, Murata S, Ohashi K. 2022. Exploration of serum biomarkers in dogs with malignant melanoma receiving anti-PD-L1 therapy and potential of COX-2 inhibition for combination therapy. Sci. Rep., 12(1):9265. doi: 10.1038/s41598-022-13484-8

 

Wada Y, Sato T, Hasegawa H, Matsudaira T, Nao N, Coler-Reilly ALG, Tasaka T, Yamauchi S, Okagawa T, Momose H, Tanio M, Kuramitsu M, Sasaki D, Matsumoto N, Yagishita N, Yamauchi J, Araya N, Tanabe K, Yamagishi M, Nakashima M, Nakahata S, Iha H, Ogata M, Muramatsu M, Imaizumi Y, Uchimaru K, Miyazaki Y, Konnai S, Yanagihara K, Morishita K, Watanabe T, Yamano Y, Saito M. 2022. RAISING is a high-performance method for identifying random transgene integration sites. Commun. Biol., 5(1):535. doi: 10.1038/s42003-022-03467-w

 

Sajiki Y, Konnai S, Okagawa T, Maekawa N, Isezaki M, Yamada S, Ito T, Sato K, Kawabata H, Logullo C, da Silva Vaz Jr I, Murata S, Ohashi K. 2022. Suppressive effects of Ixodes persulcatus sialostatin L2 against Borrelia miyamotoi-stimulated immunity. Ticks Tick Borne Dis., 13(4):101963. doi: 10.1016/j.ttbdis.2022.101963

 

Sajiki Y, Konnai S, Okagawa T, Maekawa N, Goto S, Kohara J, Nitanai A, Takahashi H, Kubota K, Takeda H, Murata S, Ohashi K. 2022. Estradiol-induced immune suppression via prostaglandin E2 during parturition in bovine leukemia virus-infected cattle. PLoS One, 17(3):e0263660. doi: 10.1371/journal.pone.0263660

 

Sato J, Murata S, Yang Z, Kaufer BB, Fujisawa S, Seo H, Maekawa N, Okagawa T, Konnai S, Osterrieder N, Parcells MS, Ohashi K. 2022. Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek’s Disease Virus on Transactivation Activity and Virulence. Viruses, 14(2):382. doi: 10.3390/v14020382

 

Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K. 2022. Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen. Parasitology, 149(1):105-115. doi: 10.1017/S0031182021001608

 

Gulay KCM, Aoshima K, Maekawa N, Suzuki T, Konnai S, Kobayashi A, Kimura T. 2022. Hemangiosarcoma cells induce M2 polarization and PD-L1 expression in macrophages. Sci. Rep., 12(1):2124. doi: 10.1038/s41598-022-06203-w

 

Noce BD, da Silva RM, de Carvalho Uhl MV, Konnai S, Ohashi K, Calixto C, Arcanjo A, de Abreu LA, de Carvalho SS, da Silva Vaz I Jr, Logullo C. 2022. Redox Imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line. J. Biol. Chem., 18:101599. doi: 10.1016/j.jbc.2022.101599

 

Ariizumi T, Murata S, Fujisawa S, Isezaki M, Sato T, Oishi E, Taneno A, Ichii O, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2022. In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens. Poult. Sci., 101(3):101638. doi: 10.1016/j.psj.2021.101638

 

Watari K, Konnai S, Okagawa T, Maekawa N, Sajiki Y, Kato Y, Suzuki Y, Murata S, Ohashi K. 2022. Enhancement of interleukin-2 production by bovine peripheral blood mononuclear cells treated with the combination of anti-programmed death-ligand 1 and cytotoxic T lymphocyte antigen 4 chimeric monoclonal antibodies. J. Vet. Med. Sci., 84(1):6-15. doi: 10.1292/jvms.21-0552

 

2021

Original articles

Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K. 2021. Characterization of a Novel Cysteine Protease Inhibitor from Poultry Red Mites: Potential Vaccine for Chickens. Vaccines, 9(12): 1472. doi: 10.3390/vaccines9121472

 

Murata S, Yamamoto E, Sakashita N, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2021. Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan. Poult. Sci., 100(11):101461. doi: 10.1016/j.psj.2021.101461

 

Fujisawa S, Murata S, Takehara M, Aoyama J, Morita A, Isezaki M, Win SY, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K. 2021. In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens. Vaccine, 39(41):6057-6066. doi: 10.1016/j.vaccine.2021.08.104

 

Ganbaatar O, Konnai S, Okagawa T, Nojima Y, Maekawa N, Ichikawa Y, Kobayashi A, Shibahara T, Yanagawa Y, Higuchi H, Kato Y, Suzuki Y, Murata S, Ohashi K. 2021. Programmed death-ligand 1 expression in swine chronic infections and enhancement of interleukin-2 production via programmed death-1/programmed death-ligand 1 blockade. Immun. Inflamm. Dis., 9(4):1573-1583. doi: 10.1002/iid3.510

 

Takeuchi H, Konnai S, Maekawa N, Takagi S, Ohta H, Sasaki N, Kim S, Okagawa T, Suzuki Y, Murata S, Ohashi K. 2021. Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression. Front. Vet. Sci., 8:656715. doi: 10.3389/fvets.2021.656715

 

Mekata H, Okagawa T, Konnai S, Miyazawa T. 2021. Molecular Epidemiology and Whole-Genome Analysis of Bovine Foamy Virus in Japan. Viruses, 13(6):1017. doi: 10.3390/v13061017

 

da Silva RM, Vital WO, Martins RS, Moraes J, Gomes H, Calixto C, Konnai S, Ohashi K, da Silva Vaz I Jr, Logullo C. 2021. Differential expression of PEPCK isoforms is correlated to Aedes aegypti oogenesis and embryogenesis. Comp. Biochem. Physiol. B Biochem. Mol. Biol., 256:110618. doi: 10.1016/j.cbpb.2021.110618

 

Sajiki Y, Konnai S, Ikenaka Y, Okagawa T, Maekawa N, Logullo C, da Silva Vaz I Jr, Murata S, Ohashi K. 2021. Prostaglandin-related immune suppression in cattle. Vet. Immunol. Immunopathol., 236:110238. doi: 10.1016/j.vetimm.2021.110238 

 

Xavier MA, Konnai S, Parizi LF, Githaka NW, Isezaki M, Goto S, Fujisawa S, Yamada S, Okagawa T, Maekawa N, Logullo C, da Silva Vaz Jr I, Murata S, Ohashi K. 2021. Cross-species reactivity of antibodies against Ixodes persulcatus ferritin 2 to Rhipicephalus microplus. Jpn. J. Vet. Res., 69(1):57-65. doi: 10.14943/jjvr.69.1.57

 

Taniguchi Y, Konnai S, Sakakibara S, Yamamoto A, Okagawa T, Maekawa N, Murata S, Ohashi K. 2021. Intrauterine infection with Mycobacterium avium subsp. paratuberculosis in pregnant cattle diagnosed with Johne's disease. Jpn. J. Vet. Res., 69(1):51-55. doi: 10.14943/jjvr.69.1.51

 

Maekawa N, Konnai S, Nishimura M, Kagawa Y, Takagi S, Hosoya K, Ohta H, Kim S, Okagawa T, Izumi Y, Deguchi T, Kato Y, Yamamoto S, Yamamoto K, Toda M, Nakajima C, Suzuki Y, Murata S, Ohashi K. 2021. PD-L1 immunohistochemistry for canine cancers and clinical benefit of anti-PD-L1 antibody in dogs with pulmonary metastatic oral malignant melanoma. npj Precis. Oncol., 5(1):10. doi: 10.1038/s41698-021-00147-6

 

Ariizumi T, Murata S, Fujisawa S, Iseszaki M, Maekawa N, Okagawa T, Sato T, Oishi E, Taneno A, Konnai S, Ohashi K. 2021. Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae). J. Vet. Med. Sci., 83(4):558-565. doi: 10.1292/jvms.20-0677

 

Tirloni L, Calvo E, Konnai S, da Silva Vaz I Jr. 2021. Editorial: The Role of Saliva in Arthropod-Host-Pathogen Relationships. Front. Cell. Infect. Microbiol. 10:630626. doi: 10.3389/fcimb.2020.63062

 

Murata S, Taniguchi A, Isezaki M, Fujisawa S, Sakai E, Taneno A, Ichii O, Ito T, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2021. Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens. Parasite, 28:9. doi: 10.1051/parasite/2021005

 

Sajiki Y, Konnai S, Nagata R, Kawaji S, Nakamura H, Fujisawa S, Okagawa T, Maekawa N, Kato Y, Suzuki Y, Murata S, Mori Y, Ohashi K. 2021. The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis. J. Vet. Med. Sci., 83(2):162-166. doi: 10.1292/jvms.20-0590

 

Kayasaki F, Okagawa T, Konnai S, Kohara J, Sajiki Y, Watari K, Ganbaatar O, Goto S, Nakamura H, Shimakura H, Minato E, Kobayashi A, Kubota M, Terasaki N, Takeda A, Noda H, Honma M, Maekawa N, Murata S, Ohashi K. 2021. Direct evidence of the preventive effect of milk replacer–based probiotic feeding in calves against severe diarrhea. Vet. Microbiol., 254:108976. doi: 10.1016/j.vetmic.2020.108976

 

Sajiki Y, Konnai S, Ikenaka Y, Gulay KCM, Kobayashi A, Parizi LF, João BC, Watari K, Fujisawa S, Okagawa T, Maekawa N, Logullo C, da Silva Vaz I Jr, Murata S, Ohashi K. 2021. Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression. Sci. Rep., 11:1063. doi: 10.1038/s41598-020-80251-y

 

Sajiki Y, Konnai S, Okagawa T, Maekawa N, Nakamura H, Kato Y, Suzuki Y, Murata S, Ohashi K. 2021. A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus. Dev. Comp. Immunol., 114:103847. doi: 10.1016/j.dci.2020.103847

 

2020

Original articles

Sajiki Y, Konnai S, Cai Z, Takada K, Okagawa T, Maekawa N, Fujisawa S, Kato Y, Suzuki Y, Murata S, Ohashi K. 2020. Enhanced Immunotherapeutic Efficacy of Anti–PD-L1 Antibody in Combination with an EP4 Antagonist. ImmunoHorizons, 4(12):837-850. doi: 10.4049/immunohorizons.2000089

 

Conradie AM, Bertzbach LD, Trimpert J, Patria JN, Murata S, Parcells MS, Kaufer BB. 2020. Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance. PLoS Pathog., 16:e1009104. doi: 10.1371/journal.ppat.1009104

 

Sajiki Y, Konnai S, Goto S, Okagawa T, Ohira K, Shimakura H, Maekawa N, Gondaira S, Higuchi H, Tajima M, Hirano Y, Kohara J, Murata S, Ohashi K. 2020. The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis.  Front. Vet. Sci., 7:609443. doi: 10.3389/fvets.2020.609443

 

Yang Z, Murata S, Fujisawa S, Takehara M, Katakura K, Hmoon MM, Win SY, Bawm S, Konnai S, Ohashi K. 2020. Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar. BMC Vet. Res., 16:453. doi: 10.1186/s12917-020-02666-z

 

Murata S, Machida Y, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2020. Genetic characterization of a Marek’s disease virus strain isolated in Japan. Virol. J., 17:186. doi: 10.1186/s12985-020-01456-1

 

Win SY, Chel HM, Hmoon MM, Htun LL, Bawm S, Win MM, Murata S, Nonaka N, Nakao R, Katakura K. 2020. Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar. Acta Tropica, 212:105719. doi: 10.1016/j.actatropica.2020.105719

 

Ganbaatar O, Konnai S, Okagawa T, Nojima Y, Maekawa N, Minato E, Kobayashi A, Ando R, Sasaki N, Miyakoshi D, Ichii O, Kato Y, Suzuki Y, Murata S, Ohashi K. 2020. PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade. PLoS One, 15(11):e0234218. doi: 10.1371/journal.pone.0234218

 

Githaka NW, Konnai S, Isezaki M, Goto S, Xavier MA, Fujisawa S, Yamada S, Okagawa T, Maekawa N, Logullo C, da Silva Vaz I Jr, Murata S, Ohashi K. 2020. Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze. Ticks Tick. Borne. Dis., 11(6):101547. doi: 10.1016/j.ttbdis.2020.101547

 

Kaneko MK, Sano M, Takei J, Asano T, Sayama Y, Hosono H, Kobayashi A, Konnai S, Kato Y. 2020. Development and Characterization of Anti-Sheep Podoplanin Monoclonal Antibodies PMab-253 and PMab-260. Monoclon. Antib. Immunodiagn. Immunother., 39(4):144-155. doi: 10.1089/mab.2020.0018

 

Takeuchi H, Konnai S, Maekawa N, Minato E, Ichikawa Y, Kobayashi A, Okagawa T, Murata S, Ohashi K. 2020. Expression Analysis of Canine CMTM6 and CMTM4 as Potential Regulators of the PD-L1 Protein in Canine Cancers. Front. Vet. Sci., 7:330. doi:10.3389/fvets.2020.00330

 

Fujisawa S, Murata S, Isezaki M, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2020.Transcriptome dynamics of blood-fed and starved poultry red mites, Dermanyssus gallinae. Parasitol. Int., 78:102156. doi:10.1016/j.parint.2020.102156

 

Goto S, Konnai S, Hirano Y, Kohara J, Okagawa T, Maekawa N, Sajiki Y, Watari K, Minato E, Kobayashi A, Gondaira S, Higuchi H, Koiwa M, Tajima M, Taguchi E, Uemura R, Yamada S, Kaneko MK, Kato Y, Yamamoto K, Toda M, Suzuki Y, Murata S, Ohashi K. 2020. Clinical efficacy of the combined treatment of anti-PD-L1 rat-bovine chimeric antibody with a COX-2 inhibitor in calves infected with Mycoplasma bovis. Jpn. J. Vet. Res., 68(2):77-90. doi: 10.14943/jjvr.68.2.77

 

Goto S, Konnai S, Hirano Y, Kohara J, Okagawa T, Maekawa N, Sajiki Y, Watari K, Minato E, Kobayashi A, Gondaira S, Higuchi H, Koiwa M, Tajima M, Taguchi E, Uemura R, Yamada S, Kaneko MK, Kato Y, Yamamoto K, Toda M, Suzuki Y, Murata S, Ohashi K. 2020. Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection. Front. Vet. Sci., 7:12. doi: 10.3389/fvets.2020.00012

 

Gondaira S, Nishi K, Tanaka T, Yamamoto T, Nebu T, Watanabe R, Konnai S, Hayashi T, Kiku Y, Okamoto M, Matsuda K, Koiwa M, Iwano H, Nagahata H, Higuchi H. 2020. Immunosuppression in cows following intramammary infusion of Mycoplasma bovis. Infect. Immun., 88(3):e00521-19. doi: 10.1128/IAI.00521-19


Parizi LF, Rangel CK, Sabadin GA, Saggin BF, Kiio I, Xavier MA, da Silva Matos R, Camargo-Mathias MI, Seixas A, Konnai S, Ohashi K, Githaka NW, da Silva Vaz I Jr. 2020. Rhipicephalus microplus cystatin as a potential cross-protective tick vaccine against Rhipicephalus appendiculatus. Ticks Tick. Borne. Dis.,11(3):101378. doi: 10.1016/j.ttbdis.2020.101378


Sajiki Y, Konnai S, Ochi A, Okagawa T, Githaka N, Isezaki M, Yamada S, Ito T, Ando S, Kawabata H, Logullo C, da Silva Vaz I Jr, Maekawa N, Murata S, Ohashi K. 2020. Immunosuppressive effects of sialostatin L1 and L2 isolated from the taiga tick Ixodes persulcatus Schulze. Ticks Tick. Borne. Dis., 11(2):101332. doi: 10.1016/j.ttbdis.2019.101332

 

2019

Original articles

Takei J, Yamada S, Konnai S, Ishinazaka T, Shimozuru M, Kaneko MK, Kato Y. 2019. PMab-241 Specifically Detects Bear Podoplanin of Lymphatic Endothelial Cells in the Lung of Brown Bear. Monoclon. Antib. Immunodiagn. Immunother., 38(6):282-284. doi: 10.1089/mab.2019.0038.

 

Watari K, Konnai S, Maekawa N, Okagawa T, Suzuki Y, Murata S, Ohashi K. 2019. Immune inhibitory function of bovine CTLA-4 and the effects of its blockade in IFN-γ production. BMC Vet. Res., 15(1):380. doi: 10.1186/s12917-019-2082-7.

 

Kato Y, Takei J, Furusawa Y, Sayama Y, Sano M, Konnai S, Kobayashi A, Harada H, Takahashi M, Suzuki H, Yamada S, Kaneko MK. 2019. Epitope Mapping of Anti-Bear Podoplanin Monoclonal Antibody PMab-247. Monoclon. Antib. Immunodiagn. Immunother., 38(5):230-233. doi: 10.1089/mab.2019.0025.

 

Inagaki H, Konnai S, Kaburagi H, Murota H, Takabatake N, Watari K, Okagawa T, Maekawa N, Murata S, Ohashi K. 2019. Vector transmission of bovine leukemia virus during summer season in Northern Hokkaido. Jpn. J. Vet. Res., 67(3):235-239. 10.14943/jjvr.67.3.235

 

Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Watari K, Minato E, Kobayashi A, Kohara J, Yamada S, Kaneko MK, Kato Y, Takahashi H, Terasaki N, Takeda A, Yamamoto K, Toda M, Suzuki Y, Murata S, Ohashi K. 2019. Prostaglandin E2-Induced Immune Exhaustion and Enhancement of Antiviral Effects by Anti-PD-L1 Antibody Combined with COX-2 Inhibitor in Bovine Leukemia Virus Infection. J. Immunol., 203(5):1313-1324. doi: 10.4049/jimmunol.1900342

 

Takei J, Furusawa Y, Yamada S, Nakamura T, Sayama Y, Sano M, Konnai S, Kobayashi A, Harada H, Kaneko MK, Kato Y. 2019. PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis. Monoclon. Antib. Immunodiagn. Immunother., 38(4):171-174. doi: 10.1089/mab.2019.0019

 

Fujisawa S, Murata S, Takehara M, Katakura K, Hmoon MM, Win SY, Ohashi K. 2019. Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar. BMC Vet. Res., 15:261. doi: 10.1186/s12917-019-2018-2

 

Yamada S, Itai S, Nakamura T, Takei J, Sano M, Konnai S, Kobayashi A, Nakagun S, Kobayashi Y, Kaneko MK, Kato Y. 2019. Immunohistochemical Analysis of the Harbor Porpoise Using Antipodoplanin Antibody PMab-237. Monoclon Antib Immunodiagn Immunother., 38(3):104-107. doi: 10.1089/mab.2019.0014

 

Takehara M, Murata S, Katakura K, Fujisawa S, Hmoon MM, Win SY, Bawn S, Htun LL, Aung YH, Win MM, Isezaki M, Maekawa N, Okagawa T, Konnai S, Ohashi K. 2019. Haematophago doius mites on poultry farms in the Republic of the Union of Myanmar. Heliyon, 5(4):e01544. doi: 10.1016/j.heliyon.2019.e01544

 

Della Noce B, Carvalho Uhl MV, Machado J, Waltero CF, de Abreu LA, da Silva RM, da Fonseca RN, de Barros CM, Sabadin G, Konnai S, da Silva Vaz I Jr, Ohashi K, Logullo C. 2019. Carbohydrate Metabolic Compensation Coupled to High Tolerance to Oxidative Stress in Ticks. Sci. Rep., 9(1):4753. doi: 10.1038/s41598-019-41036-0

 

Fujisawa S, Konnai S, Okagawa T, Maekawa N, Tanaka A, Suzuki Y, Murata S, Ohashi K. 2019. Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy. BMC Vet. Res., 15(1):68. doi: 10.1186/s12917-019-1813-0

 

2018

Original articles

Kato, Y., Yamada, S., Itai, S., Kobayashi, A., Konnai, S. and Kaneko, M. K. 2018. Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is Useful for Detecting Lymphatic Endothelial Cells by Immunohistochemical Analysis. Monoclon. Antib. Immunodiagn. Immunother., 37(6): 272-274. doi: 10.1089/mab.2018.0044

 

Kato, Y., Yamada, S., Itai, S., Kobayashi, A., Konnai, S. and Kaneko, M. K. 2018. Detection of Alpaca Podoplanin by Immunohistochemistry Using the Antibovine Podoplanin Monoclonal Antibody PMab-44. Monoclon. Antib. Immunodiagn. Immunother., 37(6): 268-271. doi: 10.1089/mab.2018.0037

 

Kato, Y., Yamada, S., Itai, S., Kobayashi, A., Konnai, S. and Kaneko, M. K. 2018. Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44. Monoclon. Antib. Immunodiagn. Immunother., 37(6): 265-268. doi: 10.1089/mab.2018.0036

 

Kato, Y, Ohishi, T., Kawada, M., Maekawa, N., Konnai, S., Itai, S., Yamada, S. and Kaneko, M. K. 2018. The mouse-canine chimeric anti-dog podoplanin antibody P38B exerts antitumor activity in mouse xenograft models. Biochem. Biophys. Rep., 17: 23-26. doi: 10.1016/j.bbrep.2018.11.005

 

Maekawa, N., Konnai, S., Balbin, M. M., Mingala, C. N., Gicana, K. R. B., Bernando, F. A. E. M., Murata, S. and Ohashi, K. 2018. Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog. Ticks. Tick. Borne. Dis., 9(2): 266-269. doi: 10.1016/j.ttbdis.2017.09.012

 

Ochirkhuu N, Konnai S, Odbileg R, Okagawa T, Murata S, Ohashi K. Identification of immuno-inhibitory molecules in Mongolian native cattle and yak. Jpn. J. Vet. Res., 66(3):177-192. doi: 10.14943/jjvr.66.3.177

 

Mekata H, Yamamoto M, Hayashi T, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J. 2018. Cattle with a low bovine leukemia virus proviral load are rarely an infectious source. Jpn. J. Vet. Res., 66(3):157-163. doi: 10.14943/jjvr.66.3.157

 

Tagawa M, Kurashima C, Takagi S, Maekawa N, Konnai S, Shimbo G, Matsumoto K, Inokuma H, Kawamoto K, Miyahara K. 2018. Evaluation of costimulatory molecules in dogs with B cell high grade lymphoma. PLoS One, 13(7):e0201222. doi: 10.1371/journal.pone.0201222

 

Yamada S, Itai S, Kaneko MK, Konnai S, Kato Y. 2018. Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1. Biochem. Biophys. Rep., 15:52-56. doi: 10.1016/j.bbrep.2018.07.002

 

Okagawa T, Konnai S, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Kohara J, Suzuki Y, Yamada S, Kato Y, Murata S, Ohashi K. Cooperation of PD-1 and LAG-3 in the exhaustion of CD4+ and CD8+ T cells during bovine leukemia virus infection. Vet. Res., 49:50. doi: 10.1186/s13567-018-0543-9

 

Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Nagata R, Kawaji S, Kagawa Y, Yamada S, Kato Y, Nakajima C, Suzuki Y, Murata S, Mori Y, Ohashi K. 2018. Prostaglandin E2 induction suppresses the Th1 immune responses in cattle with Johne's disease. Infect. Immun., 86(5):e00910-17. doi: 10.1128/IAI.00910-17

 

Mekata H, Yamamoto M, Kirino Y, Sekiguchi S, Konnai S, Horii Y, Norimine J. 2018. New hematological key for bovine leukemia virus-infected Japanese Black cattle. J. Vet. Med. Sci., 80(2):316-319. doi: 10.1292/jvms.17-0455. Epub 2018 Jan 22.

 

Seixas A, Alzugaray MF, Tirloni L, Parizi LF, Pinto AFM, Githaka NW, Konnai S, Ohashi K, Yates JR III, Termignoni C, da Silva Vaz I Jr. 2018. Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis. Ticks. Tick. Borne. Dis., 9(1):72-81. doi: 10.1016/j.ttbdis.2017.10.006. Epub 2017 Oct 16.

 

2017

Original articles

Sajiki Y, Konnai S, Nishimori A, Okagawa T, Maekawa N, Goto S, Nagano M, Kohara J, Kitano N, Takahashi T, Tajima M, Mekata H, Horii Y, Murata S, Ohashi K. 2017. Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load. J. Vet. Med. Sci. 79:2036-2039. doi: 10.1292/jvms.17-0391. Epub 2017 Nov 6.

 

Itai S, Yamada S, Kaneko MK, Harada H, Kagawa Y, Konnai S, Kato Y. 2017. Expression of Cat Podoplanin in Feline Squamous Cell Carcinomas. Monoclon. Antib. Immunodiagn. Immunother. 36:243-250. doi: 10.1089/mab.2017.0046. Epub 2017 Nov 1.

 

Ochirkhuu N, Konnai S, Odbileg R, Murata S, Ohashi K. 2017. Molecular epidemiological survey and genetic characterization of ovine gammaherpesvirus-2 in Mongolian livestock. J. Vet. Med. Sci. 79:2040-2042. doi: 10.1292/jvms.17-0203. Epub 2017 Oct 19.

 

Sharav T, Konnai S, Ochirkhuu N, Ts EO, Mekata H, Sakoda Y, Umemura T, Murata S, Chultemdorj T, Ohashi K. 2017. Detection and molecular characterization of equine infectious anemia virus in Mongolian horses. J. Vet. Med. Sci. 79:1884-1888. doi: 10.1292/jvms.17-0202. Epub 2017 Oct 11.

 

Maekawa N, Konnai S, Balbin MM, Mingala CN, Gicana KRB, Bernando FAEM, Murata S, Ohashi K. 2017. Molecular detection and phylogenetic analysis of Ehrlichia canis in a Philippine dog. Ticks Tick Borne Dis. 9:266-269. doi: 10.1016/j.ttbdis.2017.09.012. Epub 2017 Sep 20.

 

Maekawa, N., Konnai, S., Takagi, S., Kagawa, Y., Okagawa, T., Nishimori, A., Ikebuchi, R., Izumi, Y., Deguchi, T., Nakajima, C., Kato, Y., Yamamoto, K., Uemura, H., Suzuki, Y., Murata, S. and Ohashi K. 2017. A canine chimeric monoclonal antibody targeting PD-L1 and its clinical efficacy in canine oral malignant melanoma or undifferentiated sarcoma. Sci. Rep. 7:8951. (doi: 10.1038/s41598-017-09444-2.)

 

Yamada, S., Itai, S., Nakamura, T., Yanaka, M., Saidoh, N., Chang, Y. W., Handa, S., Harada, H., Kagawa, Y., Ichii, O., Konnai, S., Kaneko, M. K. and Kato, Y. 2017. PMab-52: Specific and Sensitive Monoclonal Antibody Against Cat Podoplanin for Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother. 36:224-230. doi: 10.1089/mab.2017.0027. Epub 2017 Jul 24.

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Murata, S. and Ohashi, K. 2017. Molecular Epidemiological Survey and Genetic Characterization of Anaplasma Species in Mongolian Livestock. Vector Borne Zoonotic Dis. 17:539-549. (doi:10.1089/vbz.2017.2111.)

 

Nishimori, A., Konnai, S., Okagawa, T., Maekawa, N., Goto, S., Ikebuchi, R., Nakahara, A., Chiba, Y., Ikeda, M., Murata, S. and Ohashi, K. 2017. Identification of an atypical enzootic bovine leukosis in Japan by using a novel classification of bovine leukemia based on immunophenotypic analysis. Clin. Vaccine Immunol. 24(9):e00067-17. (doi: 10.1128/CVI.00067-17.)

 

Okagawa, T., Konnai, S., Nishimori, A., Maekawa, N., Ikebuchi, R., Goto, S., Nakajima, C., Kohara, J., Ogasawara, S., Kato, Y., Suzuki, Y., Murata, S. and Ohashi, K. 2017. Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Front. Immunol. 8:50. (doi:10.3389/fimmu.2017.00650.)

 

Goto, S., Konnai, S., Okagawa, T., Nishimori, A., Maekawa, N., Gondaira, S., Higuchi, H., Koiwa, M., Tajima, M., Kohara, J., Ogasawara, S., Kato, Y., Suzuki, Y., Murata, S. and Ohashi, K. 2017. Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis. Immun. Inflamm. Dis. 5:355-363. doi: 10.1002/iid3.173. Epub 2017 May 24.

 

Yamada, S., Honma, R., Kaneko, M. K., Nakamura, T., Yanaka, M., Saidoh, N., Takagi, M., Konnai, S. and Kato, Y. 2017. Characterization of the Anti-Bovine Podoplanin Monoclonal Antibody PMab-44. Monoclon. Antib. Immunodiagn. Immunother. 36: 129-134. (doi:10.1089/mab.2017.0016.)

 

Nishimori, A., Konnai, S., Okagawa, T., Maekawa, N., Ikebuchi, R., Goto, S., Sajiki, Y., Suzuki, Y., Kohara, J., Ogasawara, S., Kato, Y., Murata, S. and Ohashi, K. 2017. In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection. PLoS One. 12: e0174916. (doi: 10.1371/journal.pone.0174916.)

 

Yamada, S., Kaneko, M. K., Nakamura, T., Ichii, O., Konnai, S. and Kato, Y. 2017. Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin. Monoclon. Antib. Immunodiagn. Immunother. 36: 77-79. (doi: 10.1089/mab.2017.0002.)

 

Rangel, C. K., Parizi, L. F., Sabadin, G. A., Costa, E.P., Romeiro, N. C., Isezaki, M., Githaka, N.W., Seixas, A., Logullo, C., Konnai, S., Ohashi, K. and da Silva Vaz I. Jr. 2017. Molecular and structural characterization of novel cystatins from the taiga tick Ixodes persulcatus. Ticks Tick Borne Dis. 8: 432-441. (doi:10.1016/j.ttbdis.2017.01.007.)

 

Pandey, G. S., Simulundu, E., Mwiinga, D., Samui, K. L., Mweene, A. S., Kajihara, M., Mangani, A., Mwenda, R., Ndebe, J., Konnai, S. and Takada, A. 2017. Clinical and subclinical bovine leukemia virus infection in a dairy cattle herd in Zambia. Arch. Virol. 162: 1051-1056. (doi: 10.1007/s00705-016-3205-0.)

 

Konnai, S., Murata, S. and Ohashi, K. 2017. Immune exhaustion during chronic infections in cattle. J. Vet. Med. Sci. 79: 1-5. (doi: 10.1292/jvms.16-0354.)

 

Costa, E. P., Façanha, A. R., Cruz, C. S., Silva, J. N., Machado, J. A., Carvalho, G. M., Fernandes, M. R., Martins, R., Campos, E., Romeiro, N. C., Githaka, N. W., Konnai, S., Ohashi, K., Vaz I. S. Jr. and Logullo, C. 2017. A novel mechanism of functional cooperativity regulation by thiol redox status in a dimeric inorganic pyrophosphatase. Biochim. Biophys. Acta. 1861(1 Pt A): 2922-2933. (doi: 10.1016/j.bbagen.2016.09.017.)

 

Machida, Y., Murata, S., Matsuyama-Kato, A., Isezaki, M., Taneno, A., Sakai, E., Konnai, S. and Ohashi, K. 2017. Isolation and purification of Gallid herpesvirus 2 strains currently distributed in Japan. J. Vet. Med. Sci. 79: 115-122. (doi:10.1292/jvms.16-0329.)

 

2016

Original articles

Honma, R., Ogasawara, S., Kaneko, M. K., Fujii, Y., Oki, H., Nakamura, T., Takagi, M., Konnai, S. and Kato, Y. 2016. PMab-44 Detects Bovine Podoplanin in Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother., 35: 186-190.

 

Ogasawara, S., Honma, R., Kaneko, M. K., Fujii, Y., Kagawa, Y., Konnai, S. and Kato, Y. 2016. Podoplanin Expression in Canine Melanoma. Monoclon. Antib. Immunodiagn. Immunother. 35: 304-306. (doi: 10.1089/mab.2016.0040.)

 

Kaneko, M. K., Honma, R., Ogasawara, S., Fujii, Y., Nakamura, T., Saidoh, N., Takagi, M., Kagawa, Y., Konnai, S. and Kato, Y. 2016. PMab-38 Recognizes Canine Podoplanin of Squamous Cell Carcinomas. Monoclon. Antib. Immunodiagn. Immunother. 35: 263-266.

 

Okagawa, T., Konnai, S., Deringer, J. R., Ueti, M. W., Scoles, G. A., Murata, S., Ohashi, K. and Brown, W. C. 2016. Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle. Infect. Immun. 84: 2779-2790. (doi: 10.1128/IAI.00278-16.)

 

Honma, R., Kaneko, M. K., Ogasawara, S., Fujii, Y., Konnai, S., Takagi, M. and Kato, Y. 2016. Specific Detection of Dog Podoplanin Expressed in Renal Glomerulus by a Novel Monoclonal Antibody PMab-38 in Immunohistochemistry. Monoclon. Antib. Immunodiagn. Immunother. 35: 212-216. (doi: 10.1089/mab.2016.0022.)

 

Maekawa, N., Konnai, S., Okagawa, T., Nishimori, A., Ikebuchi, R., Izumi, Y., Takagi, S., Kagawa, Y., Nakajima, C., Suzuki, Y., Kato, Y., Murata, S. and Ohashi, K. 2016. Immunohistochemical Analysis of PD-L1 Expression in Canine Malignant Cancers and PD-1 Expression on Lymphocytes in Canine Oral Melanoma. PLoS One. 11: e0157176. (doi: 10.1371/journal.pone.0157176.)

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Odzaya, B., Gansukh, S., Murata, S. and Ohashi, K. 2016. Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks. Arch Virol. 161: 2279-2283. (doi: 10.1007/s00705-016-2890-z.)

 

Ohira, K., Nakahara, A., Konnai, S., Okagawa, T., Nishimori, A., Maekawa, N., Ikebuchi, R., Kohara, J., Murata, S. and Ohashi, K. 2016. Bovine leukemia virus reduces anti-viral cytokine activities and NK cytotoxicity by inducing TGF-β secretion from regulatory T cells. Immun. Inflamm. Dis. 4: 52-63. (doi: 10.1002/iid3.93.)

 

Honma, R., Fujii, Y., Ogasawara, S., Oki, H., Konnai, S., Kagawa, Y., Takagi, M., Kaneko, M. K. and Kato, Y. 2016. Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis. Monoclon. Antib. Immunodiagn. Immunother. 35: 65-72. (doi: 10.1089/mab.2015.0078.)

 

Nishimori, A., Konnai, S., Ikebuchi, R., Okagawa, T., Nakahara, A., Murata, S. and Ohashi, K. 2016. Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection. J. Vet. Med. Sci. 78: 791-796. (doi: 10.1292/jvms.15-0577.)

 

Tagawa, M., Maekawa, N., Konnai, S. and Takagi, S. 2016. Evaluation of Costimulatory Molecules in Peripheral Blood Lymphocytes of Canine Patients with Histiocytic Sarcoma. PLoS One. 11: e0150030. (doi: 10.1371/journal.pone.0150030.)

 

Ochirkhuu, N., Konnai, S., Odbileg, R., Nishimori, A., Okagawa, T., Murata, S. and Ohashi, K. 2016. Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle. Arch. Virol. 161: 985-991. (doi: 10.1007/s00705-015-2676-8.)

 

Toyomane, K., Konnai, S., Niwa, A., Githaka, N., Isezaki, M., Yamada, S., Ito, T., Takano, A., Ando, S., Kawabata, H., Murata, S. and Ohashi, K. 2016. Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze. Ticks Tick Borne Dis. 7: 119-125. (doi: 10.1016/j.ttbdis.2015.09.006.)

 

2015

Original articles

Ochirkhuu, N., Konnai, S., Odbileg, R., Murata, S. and Ohashi, K. 2015. A preliminary survey of the seroprevalence of Mycobacterium avium subspecies paratuberculosis in Mongolian cattle. Jpn. J. Vet. Res. 63: 191-194.

 

Okagawa, T., Konnai, S., Nishimori, A., Ikebuchi, R., Mizorogi, S., Nagata, R., Kawaji, S., Tanaka, S., Kagawa, Y., Murata, S., Mori, Y. and Ohashi, K. 2015. Bovine Immunoinhibitory Receptors Contribute to Suppression of Mycobacterium avium subsp. paratuberculosis-Specific T-Cell Responses. Infect. Immun. 84: 77-89. (doi: 10.1128/IAI.01014-15.)

 

Duran, P. L., Padiernos, R. B., Abella, E. A., Konnai, S. and Mingala, C. N. 2015. Molecular characterization of T-cell immunoglobulin mucin domain-3 and Galectin-9 genes of swamp- and riverine-type water buffaloes. Int. J. Immunogenet. 42: 469-78. (doi: 10.1111/iji.12235.)

 

Ochirkhuu, N., Konnai, S., Mingala, C. N., Okagawa, T., Villanueva, M., Pilapil, F. M., Murata, S. and Ohashi, K. 2015. Molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in Luzon Island, the Philippines. Vet. Parasitol. 212: 161-167. (doi: 10.1016/j.vetpar.2015.05.019.)

 

Gomes, H., Moraes, J., Githaka, N., Martins, R., Isezaki, M., Vaz, Ida. S. Jr., Logullo, C., Konnai, S. and Ohashi, K. 2015. Vaccination with cyclin-dependent kinase tick antigen confers protection against Ixodes infestation. Vet. Parasitol. 211: 266-273. (doi: 10.1016/j.vetpar.2015.05.022.)

 

Nakata, H., Nakayama, S. M., Ikenaka, Y., Mizukawa, H., Ishii, C., Yohannes, Y. B., Konnai, S., Darwish, W. S. and Ishizuka, M. 2015. Metal extent in blood of livestock from Dandora dumping site, Kenya: Source identification of Pb exposure by stable isotope analysis. Environ. Pollut. 205: 8-15. (doi: 10.1016/j.envpol.2015.05.003.)

 

Mekata, H., Sekiguchi, S., Konnai, S., Kirino, Y., Horii, Y. and Norimine, J. 2015. Horizontal transmission and phylogenetic analysis of bovine leukemia virus in two districts of Miyazaki, Japan. J. Vet. Med. Sci. 77: 1115-1120. (doi: 10.1292/jvms.14-0624.)

 

Parizi, L. F., Sabadin, G. A., Alzugaray, M. F., Seixas, A., Logullo, C., Konnai, S., Ohashi, K., Masuda, A. and da Silva, Vaz, I. Jr. 2015. Rhipicephalus microplus and Ixodes ovatus cystatins in tick blood digestion and evasion of host immune response. Parasit. Vectors. 8: 122. (doi: 10.1186/s13071-015-0743-3.)

 

Mekata, H., Murata, S., Mingala, C. N., Ohashi, K. and Konnai, S.2015. Expression of regulatory dendritic cell-related cytokines in cattle experimentally infected with Trypanosoma evansi. J. Vet. Med. Sci. 77: 1017-1019. (doi: 10.1292/jvms.15-0066.)

 

Gondaira, S., Higuchi, H., Iwano, H., Nakajima, K., Kawai, K., Hashiguchi, S., Konnai, S. and Nagahata, H. 2015. Cytokine mRNA profiling and the proliferative response of bovine peripheral blood mononuclear cells to Mycoplasma bovis. Vet Immunol Immunopathol. 165: 45-53. (doi: 10.1016/j.vetimm.2015.03.002.)

 

Murase, Y., Konnai, S., Yamada, S., Githaka, N., Isezaki, M., Ito, T., Takano, A., Ando, S., Kawabata, H., Murata, S. and Ohashi, K. 2015. An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes. Insect Biochem. Mol. Biol. 60: 59-67. (doi: 10.1016/j.ibmb.2015.01.010.)

 

Suzuki, S., Konnai, S., Okagawa, T., Ikebuchi, R., Nishimori, A., Kohara, J., Mingala, C. N., Murata, S. and Ohashi, K. 2015. Increased expression of the regulatory T cell-associated marker CTLA-4 in bovine leukemia virus infection. Vet. Immunol. Immunopathol. 163: 115-124. (doi: 10.1016/j.vetimm.2014.10.006.)

 

da Silva, R. M., Noce, B. D., Waltero, C. F., Costa, E. P., de Abreu, L. A., Githaka, N. W., Moraes, J., Gomes, H. F., Konnai, S., Vaz, Ida, S. Jr., Ohashi, K. and Logullo, C. 2015. Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26. Int. J. Mol. Sci. 16: 1821-1839. (doi: 10.3390/ijms16011821.)

 

Mekata, H., Sekiguchi, S., Konnai, S., Kirino, Y., Honkawa, K., Nonaka, N., Horii, Y. and Norimine, J. 2015. Evaluation of the natural perinatal transmission of bovine leukaemia virus. Vet. Rec. 176: 254. (doi: 10.1136/vr.102464.)

 

Kakinuma, S., Izawa, T., Matsuda, K., Konnai, S., Maeda, Y. and Ohtsuka, H. 2015. Cellular immune response to interferon-tau in peripheral blood mononuclear cells of Japanese black cattle with bovine leukemia virus infection. Acta Vet. (Beograd), 65: 287–296.

 

2014

Original articles

Takano A, Toyomane K, Konnai S, Ohashi K, Nakao M, Ito T, Andoh M, Maeda K, Watarai M, Sato K, Kawabata H. Tick surveillance for relapsing fever spirochete Borrelia miyamotoi in Hokkaido, Japan. PLoS One. 2014, 9(8):e104532. doi: 10.1371/journal.pone.0104532

 

Lee K, Takano A, Taylor K, Sashika M, Shimozuru M, Konnai S, Kawabata H, Tsubota T. A relapsing fever group Borrelia sp. similar to Borrelia lonestari found among wild sika deer (Cervus nippon yesoensis) and Haemaphysalis spp. ticks in Hokkaido, Japan. Ticks Tick Borne Dis. 2014, 5(6):841-847. doi: 10.1016/j.ttbdis.2014.06.006

 

Sato K, Takano A, Konnai S, Nakao M, Ito T, Koyama K, Kaneko M, Ohnishi M, Kawabata H. Human infections with Borrelia miyamotoi, Japan. Emerg. Infect. Dis. 2014, 20(8):1391-1393. doi: 10.3201/eid2008.131761

 

Maekawa N, Konnai S, Ikebuchi R, Okagawa T, Adachi M, Takagi S, Kagawa Y, Nakajima C, Suzuki Y, Murata S, Ohashi K. Expression of PD-L1 on canine tumor cells and enhancement of IFN-γ production from tumor-infiltrating cells by PD-L1 blockade. PLoS One. 2014, 9(6):e98415. doi: 10.1371/journal.pone.0098415

 

Nishimori A, Konnai S, Ikebuchi R, Okagawa T, Nakajima C, Suzuki Y, Mingala CN, Murata S, Ohashi K. Identification and characterization of bovine programmed death-ligand 2. Microbiol. Immunol. 2014, 58(7):388-397. doi: 10.1111/1348-0421.12160

 

Ikebuchi R, Konnai S, Okagawa T, Nishimori A, Nakahara A, Murata S, Ohashi K. Differences in cellular function and viral protein expression between IgMhigh and IgMlow B-cells in bovine leukemia virus-infected cattle. J. Gen. Virol. 2014, 95(Pt 8):1832-1842. doi: 10.1099/vir.0.065011-0

 

Hidano A, Konnai S, Yamada S, Githaka N, Isezaki M, Higuchi H, Nagahata H, Ito T, Takano A, Ando S, Kawabata H, Murata S, Ohahsi K. Suppressive effects of neutrophil by Salp16-like salivary gland proteins from Ixodes persulcatus Schulze tick. Insect Mol. Biol. 2014, 23(4):466-474. doi: 10.1111/imb.12101

 

Githaka N, Konnai S, Bishop R, Odongo D, Lekolool I, Kariuki E, Gakuya F, Kamau L, Isezaki M, Murata S, Ohashi K. Identification and sequence characterization of novel Theileria genotypes from the waterbuck (Kobus defassa) in a Theileria parva-endemic area in Kenya. Vet. Parasitol. 2014, 202(3-4):180-193. doi: 10.1016/j.vetpar.2014.02.056

 

Ali A, Tirloni L, Isezaki M, Seixas A, Konnai S, Ohashi K, da Silva Vaz Junior I, Termignoni C. Reprolysin metalloproteases from Ixodes persulcatus, Rhipicephalus sanguineus and Rhipicephalus microplus ticks. Exp. Appl. Acarol. 2014, 63(4):559-578. doi: 10.1007/s10493-014-9796-9

 

Matsuyama-Kato A, Murata S, Isezaki M, Takasaki S, Kano R, Konnai S, Ohashi K. Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek's disease virus. Arch. Virol. 2014, 159(8):2123-2126. doi: 10.1007/s00705-014-2021-7

 

Ikebuchi R, Konnai S, Okagawa T, Yokoyama K, Nakajima C, Suzuki Y, Murata S, Ohashi K. Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes. Immunology. 2014, 142(4):551-561. doi: 10.1111/imm.12243

 

2013

Original articles

Ooshiro M, Konnai S, Katagiri Y, Afuso M, Arakaki N, Tsuha O, Murata S, Ohashi K. Horizontal transmission of bovine leukemia virus from lymphocytotic cattle, and beneficial effects of insect vector control. Vet. Rec. 2013, 173(21):527. doi: 10.1136/vr.101833
 
Gomes H, Romeiro NC, Braz GR, de Oliveira EA, Rodrigues C, da Fonseca RN, Githaka N, Isezaki M, Konnai S, Ohashi K, da Silva Vaz I Jr, Logullo C, Moraes J. Identification and structural-functional analysis of cyclin-dependent kinases of the cattle tick Rhipicephalus (Boophilus) microplus. PLoS One. 2013, 8(10):e76128. doi: 10.1371/journal.pone.0076128

 
Parizi LF, Githaka NW, Acevedo C, Benavides U, Seixas A, Logullo C, Konnai S, Ohashi K, Masuda A, da Silva Vaz I Jr. Sequence characterization and immunogenicity of cystatins from the cattle tick Rhipicephalus (Boophilus) microplus. Ticks Tick Borne Dis. 2013, 4(6):492-499. doi: 10.1016/j.ttbdis.2013.06.005
 
Suzuki S, Konnai S, Okagawa T, Ikebuchi R, Shirai T, Sunden Y, Mingala CN, Murata S, Ohashi K. Expression analysis of Foxp3 in T cells from bovine leukemia virus infected cattle. Microbiol. Immunol. 2013, 57(8):600-604. doi: 10.1111/1348-0421.12073
 
Ikebuchi R, Konnai S, Okagawa T, Yokoyama K, Nakajima C, Suzuki Y, Murata S, Ohashi K. Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro. Vet. Res. 2013, 44:59. doi: 10.1186/1297-9716-44-59.

 
Githaka N, Konnai S, Skilton R, Kariuki E, Kanduma E, Murata S, Ohashi K. Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya. Parasitol. Int. 2013, 62(5):448-453. doi: 10.1016/j.parint.2013.06.002

 
Imamura S, Nakamizo M, Kawanishi M, Nakajima N, Yamamoto K, Uchiyama M, Hirano F, Nagai H, Kijima M, Ikebuchi R, Mekata H, Murata S, Konnai S, Ohashi K. Bovine whole-blood culture as a tool for the measurement of endotoxin activities in Gram-negative bacterial vaccines. Vet Immunol Immunopathol. 2013, 153(1-2):153-158. doi: 10.1016/j.vetimm.2013.02.002
 
Taylor KR, Takano A, Konnai S, Shimozuru M, Kawabata H, Tsubota T. Differential tick burdens may explain differential Borrelia afzelii and Borrelia
garinii infection rates among four, wild, rodent species in Hokkaido, Japan. J. Vet. Med. Sci. 2013, 75(6):785-790. doi: 10.1292/jvms.12-0439
 
Mekata H, Konnai S, Mingala CN, Abes NS, Gutierrez CA, Dargantes AP, Witola WH, Inoue N, Onuma M, Murata S, Ohashi K. Isolation, cloning, and pathologic analysis of Trypanosoma evansi field isolates. Parasitol. Res. 2013, 112(4):1513-1521. doi: 10.1007/s00436-013-3297-3

 
Murata S, Hashiguchi T, Hayashi Y, Yamamoto Y, Matsuyama-Kato A, Takasaki S, Isezaki M, Onuma M, Konnai S, Ohashi K. Characterization of Meq proteins from field isolates of Marek's disease virus in Japan. Infect. Genet. Evol. 2013, 16:137-143. doi: 10.1016/j.meegid.2012.12.032
 
de Abreu LA, Calixto C, Waltero CF, Della Noce BP, Githaka NW, Seixas A, Parizi LF, Konnai S, Vaz Ida S, Ohashi K, Logullo C. The conserved role of the AKT/GSK3 axis in cell survival and glycogen metabolism in Rhipicephalus (Boophilus) microplus embryo tick cell line BME26. Biochim. Biophys. Acta. 2013, 1830(3):2574-2582. doi: 10.1016/j.bbagen.2012.12.016

 
Imamura S, Konnai S, Yamada S, Parizi LF, Githaka N, Vaz Ida S Jr, Murata S, Ohashi K. Identification and partial characterization of a gut Rhipicephalus appendiculatus cystatin. Ticks Tick Borne Dis. 2013, 4(1-2):138-144. doi: 10.1016/j.ttbdis.2012.11.001

 
Taylor KR, Takano A, Konnai S, Shimozuru M, Kawabata H, Tsubota T. Borrelia miyamotoi infections among wild rodents show age and month independence and correlation with Ixodes persulcatus larval attachment in Hokkaido, Japan. Vector Borne Zoonotic Dis. 2013, 13(2):92-97. doi: 10.1089/vbz.2012.1027

 
Konnai S, Suzuki S, Shirai T, Ikebuchi R, Okagawa T, Sunden Y, Mingala CN, Onuma M, Murata S, Ohashi K. Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus. Comp. Immunol. Microbiol. Infect. Dis. 2013, 36(1):63-69. doi: 10.1016/j.cimid.2012.09.005

 
Murase Y, Konnai S, Githaka N, Hidano A, Taylor K, Ito T, Takano A, Ando S, Kawabata H, Tsubota T, Murata S, Ohashi K. Prevalence of Lyme borrelia in Ixodes persulcatus ticks from an area with a confirmed case of Lyme disease. J. Vet. Med. Sci. 2013, 75(2):215-218. doi: 10.1292/jvms.12-0211

 

2012

Original articles

Kohara J, Nishikura Y, Konnai S, Tajima M, Onuma M. Effects of interferon-tau on cattle persistently infected with bovine viral diarrhea virus. Jpn. J. Vet. Res. 2012, 60(2-3): 63-70. doi: 10.14943/jjvr.60.2-3.63
 
Murase Y, Konnai S, Githaka N, Hidano A, Taylor K, Ito T, Takano A, Ando S, Kawabata H, Tsubota T, Murata S, Ohashi K. Prevalence of Lyme borrelia in Ixodes persulcatus ticks from an area with a confirmed case of Lyme disease. J. Vet. Med. Sci. 2013, 75(2): 215-218. doi: 10.1292/jvms.12-0211
 
Takano A, Sugimori C, Fujita H, Kadosaka T, Taylor KR, Tsubota T, Konnai S, Tajima T, Sato K, Watanabe H, Ohnishi M, Kawabata H. A novel relapsing fever Borrelia sp. infects the salivary glands of the molted hard tick, Amblyomma geoemydae. Ticks Tick Borne Dis. 2012, 3(4):259-261. doi: 10.1016/j.ttbdis.2012.06.003
 
Okagawa T, Konnai S, Mekata H, Githaka N, Suzuki S, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K. Transcriptional profiling of inflammatory cytokine genes in African buffaloes (Syncerus caffer) infected with Theileria parva. Vet. Immunol. Immunopathol. 2012, 148(3-4):373-379. doi: 10.1016/j.vetimm.2012.06.015
 
Ikenaka Y, Nakayama SM, Muroya T, Yabe J, Konnai S, Darwish WS, Muzandu K, Choongo K, Mainda G, Teraoka H, Umemura T, Ishizuka M. Effects of environmental lead contamination on cattle in a lead/zinc mining area: changes in cattle immune systems on exposure to lead in vivo and in vitro. Environ. Toxicol. Chem. 2012, 31(10):2300-2305. doi: 10.1002/etc.1951
 
Githaka N, Konnai S, Kariuki E, Kanduma E, Murata S, Ohashi K. Molecular detection and characterization of potentially new Babesia and Theileria species/variants in wild felids from Kenya. Acta Trop. 2012, 124(1):71-78. doi: 10.1016/j.actatropica.2012.06.013
 
Parizi LF, Githaka NW, Logullo C, Konnai S, Masuda A, Ohashi K, da Silva Vaz I Jr. The quest for a universal vaccine against ticks: cross-immunity insights. Vet. J. 2012, 194(2):158-165. doi: 10.1016/j.tvjl.2012.05.023
 
Okagawa T, Konnai S, Ikebuchi R, Suzuki S, Shirai T, Sunden Y, Onuma M, Murata S, Ohashi K. Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection. Vet. Res. 2012, 43:45. doi: 10.1186/1297-9716-43-45
 
Matsuyama-Kato A, Murata S, Isezaki M, Kano R, Takasaki S, Ichii O, Konnai S, Ohashi K. Molecular characterization of immunoinhibitory factors PD-1/PD-L1 in chickens infected with Marek's disease virus. Virol. J. 2012, 9:94. doi: 10.1186/1743-422X-9-94
 
Konnai S, Yamada S, Imamura S, Nishikado H, Githaka N, Ito T, Takano A, Kawabata H, Murata S, Ohashi K. Identification of TROSPA homologue in Ixodes persulcatus Schulze, the specific vector for human Lyme borreliosis in Japan. Ticks Tick Borne Dis. 2012, 3(2):75-77. doi: 10.1016/j.ttbdis.2012.02.001
 
Mekata H, Konnai S, Mingala CN, Abes NS, Gutierrez CA, Dargantes AP, Witola WH, Inoue N, Onuma M, Murata S, Ohashi K. Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection. Parasite Immunol. 2012, 34(6):318-329. doi: 10.1111/j.1365-3024.2012.01362.x.
 
Suzuki S, Konnai S, Okagawa T, Githaka NW, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K. Molecular cloning and characterization of Th1 and Th2 cytokines of African buffalo (Syncerus caffer). Int. J. Immunogenet. 2012, 39(2):170-182. doi: 10.1111/j.1744-313X.2011.01062.x
 
Murata S, Hayashi Y, Kato A, Isezaki M, Takasaki S, Onuma M, Osa Y, Asakawa M, Konnai S, Ohashi K. Surveillance of Marek's disease virus in migratory and sedentary birds in Hokkaido, Japan. Vet. J. 2012 Jun;192(3):538-40. doi: 10.1016/j.tvjl.2011.07.006

 

2011

Original articles

Shirai T, Konnai S, Ikebuchi R, Okagawa T, Suzuki S, Sunden Y, Onuma M, Murata S, Ohashi K. Molecular cloning of bovine lymphocyte activation gene-3 and its expression characteristics in bovine leukemia virus-infected cattle. Vet. Immunol. Immunopathol. 2011,144(3-4):462-467. doi: 10.1016/j.vetimm.2011.08.018
 
Takano A, Fujita H, Kadosaka T, Konnai S, Tajima T, Watanabe H, Ohnishi M, Kawabata H. Characterization of reptile-associated Borrelia sp. in the vector tick, Amblyomma geoemydae, and its association with Lyme disease and relapsing fever Borrelia spp. Environ. Microbiol. Rep. 2011, 3(5):632-637. doi: 10.1111/j.1758-2229.2011.00280.x.
 
Ikebuchi R, Konnai S, Shirai T, Sunden Y, Murata S, Onuma M, Ohashi K. Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade. Vet. Res. 2011, 42:103. doi: 10.1186/1297-9716-42-103
 
Murata S, Hayashi Y, Kato A, Isezaki M, Takasaki S, Onuma M, Osa Y, Asakawa M, Konnai S, Ohashi K. Surveillance of Marek's disease virus in migratory and sedentary birds in Hokkaido, Japan. Vet. J. 2012, 192(3):538-540. doi: 10.1016/j.tvjl.2011.07.006
 
Murata S, Okada T, Kano R, Hayashi Y, Hashiguchi T, Onuma M, Konnai S, Ohashi K. Analysis of transcriptional activities of the Meq proteins present in highly virulent Marek's disease virus strains, RB1B and Md5. Virus. Genes. 2011, 43(1):66-71. doi: 10.1007/s11262-011-0612-x
 
Takano A, Nakao M, Masuzawa T, Takada N, Yano Y, Ishiguro F, Fujita H, Ito T, Ma X, Oikawa Y, Kawamori F, Kumagai K, Mikami T, Hanaoka N, Ando S, Honda N, Taylor K, Tsubota T, Konnai S, Watanabe H, Ohnishi M, Kawabata H. Multilocus sequence typing implicates rodents as the main reservoir host of human-pathogenic Borrelia garinii in Japan. J. Clin. Microbiol. 2011, 49(5):2035-2039. doi: 10.1128/JCM.02544-10
 
Murase Y, Konnai S, Hidano A, Githaka NW, Ito T, Takano A, Kawabata H, Ato M, Tajima T, Tajima M, Onuma M, Murata S, Ohashi K. Molecular detection of Anaplasma phagocytophilum in cattle and Ixodes persulcatus ticks. Vet. Microbiol. 2011, 149(3-4):504-507. doi: 10.1016/j.vetmic.2010.11.025
 
Konnai S, Nishikado H, Yamada S, Imamura S, Ito T, Onuma M, Murata S, Ohashi K. Molecular identification and expression analysis of lipocalins from blood feeding taiga tick, Ixodes persulcatus Schulze. Exp. Parasitol. 2011, 127(2):467-474. doi: 10.1016/j.exppara.2010.10.002
 
Mingala CN, Konnai S, Ikebuchi R, Ohashi K. Characterization of CTLA-4, PD-1 and PDL-1 of swamp and riverine type water buffaloes. Comp. Immunol. Microbiol. Infect. Dis. 2011, 34(1):55-63. doi: 10.1016/j.cimid.2010.02.002